Validierung der Inkubationsbedingungen
In-situ-Studie zu Abklatschplatten aus dem mikrobiologischen Umgebungsmonitoring
Originale
Key Words Inkubationsbedingungen | Abklatschplatten | Schaukelinkubation | In-situ-Studie | Umgebungsmonitoring
Abstract
In situ Study for Incubation Conditions for Contact Plates from Microbiological Environmental Monitoring
The current study investigates different incubation conditions for agar plates from the microbiological environmental monitoring on the growth of aerobic mesophilic microorganisms and molds. The study was performed using naturally contaminated surfaces. Four different incubation conditions were tested. Additionally, the effect of a hold time step of the used agar plates for 3 days at room temperature before incubation was investigated. Neither the incubation condition nor the hold time at room temperature had a significant effect on the total aerobic microbial count. However, the hold time significantly increased the numbers of molds found, at least in 3 out of 4 incubations conditions. This effect probably relied on the increased incubation time due to the hold time step. In conclusion, the current study shows the minimal effect of the different investigated incubation conditions on the microbial counts of agar plates from the environmental monitoring.
Korrespondenz:
Dr. phil. II Marcel Goverde, Melchtalstr. 21, 4102 Binningen (Schweiz); e-mail: goverde@mgp-consulting.ch
Zusammenfassung
In der vorliegenden Studie wurde der Einfluss verschiedener Inkubationsbedingungen auf die Gesamtkeimzahl aerober, mesophiler Mikroorganismen und Schimmelpilze beim mikrobiologischen Umgebungsmonitoring untersucht. Dazu wurden natürlich kontaminierte Oberflächen bemustert. Es wurden insgesamt 4 verschiedene Inkubationsbedingungen betrachtet.
