Privatsphäre-Einstellungen

Impedanzmessung zur Bestimmung der Luftkeimzahl

Katrin Husmann a , Bernd Wiedemann b und Klaus-Jürgen Steffens a

Abteilung Pharmazeutische Technologie a und Pharmazeutische Mikrobiologie b des Pharmazeutischen Institutes der Rheinischen Friedrich-Wilhelms-Universität, Bonn

Impedance Measurement for the Determination of Airborne Viable Germs

The monitoring of airborne viable germs in pharmaceutical cleanrooms is a much more serious problem than the particle counting in the air. With conventional methods the results of an analytical detection are only available after five days. With the impedance method, a rapid and automatical technology, exists an alternative to conventional microbiological methods. The method already offers the possibility to detect one germ per analysis, so that it is possible to control rooms of cleanroom grade A, The maximum germ safe load is one germ per m³ . For the detection of one germ with impedance measurements between 12 and 20 hours for bacteria and about 90 hours for fungi are necessary. It is possible to reduce the total time by optimisation. Therefore, 10 germs are sampled instead of one germ. It is possible to reduce the total time for detection of bacteria by one or two hours and for detection of fungi by approximately 15 hours if the germ seed is 50.

Key Words Gesamtzeit-Optimierung · Impedanzmessung · Keimzahlbestimmung