Second-Derivative Spectrophotometric Analysis of Lidocaine and Hydrocortisone Acetate in Suppositories Mirjana Medenicaa, Darko Ivanovicb, Slavko Markovicc, Andjelija Malenovicb, and Biljana Jancicb
Institute of Physical Chemistrya and Institute of Pharmaceutical Chemistry and Drug Analysisb, Faculty of Pharmacy, and Institute of Pharmacy of Serbiac, Belgrade (Yugoslavia)
In this paper the second-derivative spectrophotometric method for the simultaneous determination of lidocaine (CAS 137-58-6) and hydrocortisone acetate (CAS 50-03-3) in suppositories is described. One suppository contains 60 mg of lidocaine and 5 mg of hydrocortisone acetate. Optimal conditions for the quantitative analysis were settled. The second-derivative spectra were recorded in the range from 210 to 380 nm against methanol. Determination of lidocaine was performed at 256.0 nm and hydrocortisone acetate at 260.5 nm, using the “zero crossing” method. The results obtained show that there was no interference of ingredients matrix under the mentioned experimental conditions. The method is simple, rapid and precise and can be used in a routine analysis of such pharmaceutical dosage forms.
Key words CAS 50-03-3 • CAS 137-58-6 • Hydrocortisone acetate • Lidocaine • Second-derivative spectrophotometry, zero-crossing method
