Protein-Reactive Extractables

Rubrik: Originale

(Treffer aus pharmind, Nr. 01, Seite 104 (2018))

Haep R | Stratmann A | Watt S | Martens L

Protein-Reactive Extractables / A Screening Assay Which Adds Additional Value to the Traditional E/L Workflow · Haep R, Stratmann A, Watt S, Martens L · A&M STABTEST GmbH, Bergheim und
Extractable Leachable Product Quality Research Institute (PQRI) protein-reactive rubber oligomer polysulfanes Most protein-based biopharmaceutical drug products with market authorization to date are administered parenterally and have to be formulated accordingly – either as a liquid for injection or as a lyophilisate which has to be reconstituted with a suitable diluent prior to administration. If the stability of the drug product permits, the formulation favored by the industry is a liquid for injection which is easier to develop, more cost effective to manufacture and easier as well as safer to use for healthcare professionals. For these products, glass vials with rubber ...

Three-dimensional in vitro Tumor Models as an Alternative for Animal Models in Preclinical Studies

Rubrik: Übersichten

(Treffer aus pharmind, Nr. 04, Seite 675 (2013))

Stratmann A | Dandekar G | Nietzer S

Three-dimensional in vitro Tumor Models as an Alternative for Animal Models in Preclinical Studies / Part 2*Part 1 see Pharm. Ind. 2013;75(3):485-489. · Stratmann A, Dandekar G, Nietzer S · Chair of Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, Würzburg, Germany
Cancer Cell lines Drug screening In vitro model Tissue engineering Tumor model The limits of tumor spheroids led to many attempts to provide a 3D culture setting with advanced architecture and an improved context for coculture by introducing an element providing extracellular structure. Such elements are called either matrices or scaffolds. For standardization purposes, it has been proposed to refer to hydrogels as matrices, and to cellular solids as scaffolds. [ 75 ]. Both are generally composed of extracellular matrix components, since these have been shown to maintain the original cell function in vitro. Next to gels [ 76 ], ...

Three-dimensional in vitro Tumor Models as an Alternative for Animal Models in Preclinical Studies

Rubrik: Übersichten

(Treffer aus pharmind, Nr. 03, Seite 485 (2013))

Stratmann A | Dandekar G | Nietzer S

Three-dimensional in vitro Tumor Models as an Alternative for Animal Models in Preclinical Studies / Teil 1 · Stratmann A, Dandekar G, Nietzer S · Chair of Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, Würzburg, Germany
Cancer Cell lines Drug screening In vitro model Tissue engineering Tumor model For over 60 years, human cancer is being investigated by using various in vivo or in vitro models [ 1 , 2 ]. In 1955, the US National Cancer Institute (NCI) started the first large-scale program for drug discovery, during which the effect of various substances on mouse lymphoma models was tested in order to detect new chemotherapeutic agents. Drugs detected or developed during this program, such as anti-mitotic vinca alkaloids, actinomycin D and platinum salts, are still commonly used today [ 1 ]. Few years later, in ...